| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.071234 |
| Chromosome: | chromosome 12 |
| Location: | 4084487 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCAATTGTGGTTTACACTTGTGCCCGAAGTGGGAAGATCTGTCCACTCG |
| Internal bar code: | GTATGAGATCTAGTCGGTTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6688 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 76 |
| LEAP-Seq n unique pos: | 114 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTTGCGACTTAGCGAACA |
| Suggested primer 2: | TAATCGTGCGACTAGCCGAC |