| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.071238 |
| Chromosome: | chromosome 14 |
| Location: | 1676942 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g618820 | CSB57 | (1 of 70) PF07282 - Putative transposase DNA-binding domain (OrfB_Zn_ribbon); {"Probable transposon-derived protein of Chlamydomonas-Specific family B | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCTAACGCAGCGAGGCTTTCCTCGCGCGTGACCGCCACGCCACCGGCT |
| Internal bar code: | GTTTAAGGTAGGGTCCCTCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1142 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCTGCATTGTGTGTTTCA |
| Suggested primer 2: | GCTTGTCCAGCTTTTCCGTG |