| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.071240 |
| Chromosome: | chromosome 10 |
| Location: | 2861973 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g439400 | GAT1 | Glutamyl tRNA amidotransferase, subunit A; (1 of 1) K02433 - aspartyl-tRNA(Asn)/glutamyl-tRNA(Gln) amidotransferase subunit A (gatA, QRSL1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTAATGTAGCCCCCTACTCTCAACCCCGCTGCTTCTCTGCATCCACCT |
| Internal bar code: | GTAGACGTCGTTTACCCATTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 319 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTACAAGCTGGGCGAGAAG |
| Suggested primer 2: | CGCCCCATCACTCAGCATAT |