| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.071242 |
| Chromosome: | chromosome 16 |
| Location: | 5843641 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679800 | (1 of 2) PTHR17630//PTHR17630:SF43 - DIENELACTONE HYDROLASE // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGAATCGCCAGCCGGATCGGCACGGAAGTTGCAATGCCTCAACCAAAC |
| Internal bar code: | TGGCAAGGGTTAATGTTGGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2792 |
| LEAP-Seq percent confirming: | 98.4127 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCATGGACCCCTCACATG |
| Suggested primer 2: | ACGATGTGCAGTCAAGACGT |