Insertion junction: CLIP2.071243_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.071243
Chromosome:chromosome 12
Location:1682135
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre12.g486350 STPK3,STK3 (1 of 3) K07359 - calcium/calmodulin-dependent protein kinase kinase (CAMKK); Serine/threonine protein kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCTGGTGCCAGTACGGATGAGTTGTTGTGGCTCAAGAGGTGCAGGTGG
Internal bar code:TGGAGCATTTATACAGGTTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:783
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TGAGTTGATGGAGCGGATGC
Suggested primer 2:CCGCCTCGTGTCTCTCAATT