| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.071253 |
| Chromosome: | chromosome 4 |
| Location: | 2685956 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223350 | CYG62 | (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAATTAAGATAAGCCCGGCGATTGCGAGGGTAAAAACGATCTGTCGCATT |
| Internal bar code: | GTACTCTTACGACGAGCTTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2836 |
| LEAP-Seq percent confirming: | 79.3103 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAAGCCATTCAAGACCGGC |
| Suggested primer 2: | ACTGGATAGAGAGCGAGGCA |