| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.071256 |
| Chromosome: | chromosome 5 |
| Location: | 283387 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g243353 | (1 of 14) PF13540 - Regulator of chromosome condensation (RCC1) repeat (RCC1_2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACAATACGGGGATTGGGGAGGCGCTGAAATGGAACTCGCCTCAATTTA |
| Internal bar code: | TCGTATGTTGTAAACCTCAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2086 |
| LEAP-Seq percent confirming: | 65.2174 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGAATACGAACCCACACGT |
| Suggested primer 2: | TGTCACTGTCTCTGCTGCTG |