| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.071259 |
| Chromosome: | chromosome 4 |
| Location: | 2315978 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g220768 | (1 of 781) IPR000104 - Antifreeze protein, type I | gene_edge/mRNA_edge/CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGAGCTCGCAATATAGCAATAGTAGCCAGGGCATCTTGACCGAGGCCG |
| Internal bar code: | GGCGCCCGGTTCTATGCCATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 399 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGACGGTGGTGTCTGTTC |
| Suggested primer 2: | GTGTGGTGTTTCGACTGTGC |