| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.071277 |
| Chromosome: | chromosome 9 |
| Location: | 6198337 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g413350 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACCCAGCTCGCTCGTGTCTCTTCCTTTTCTCTCCAAACTAGCACCGGA |
| Internal bar code: | GTGTTAGTACAAAGTATTCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2039 |
| LEAP-Seq percent confirming: | 86.6667 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGGGTTGAAGTGAGGAGG |
| Suggested primer 2: | AACCACCGTATTGGACACCC |