| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.071284 |
| Chromosome: | chromosome 12 |
| Location: | 2455443 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g507559 | HSC20,HSC20B | (1 of 1) K04082 - molecular chaperone HscB (hscB, HSCB, HSC20); Co-chaperone, HscB-like | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCAGTAGAGCAGTAGTGGCATGACTTTGGCTTGGGTTGGCCCGGCTGG |
| Internal bar code: | TGTCGACTATTTCCACAGTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5329 |
| LEAP-Seq percent confirming: | 41.8367 |
| LEAP-Seq n confirming: | 82 |
| LEAP-Seq n nonconfirming: | 114 |
| LEAP-Seq n unique pos: | 196 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGCATGCCCTTTTGCTT |
| Suggested primer 2: | ACACACACACACACACAGGT |