Insertion junction: CLIP2.071295_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.071295
Chromosome:chromosome 2
Location:5884530
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre02.g117410 (1 of 5) PF03992 - Antibiotic biosynthesis monooxygenase (ABM) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGTCTTCTTGAGGTCAAACACCAGGTTGCCCTTCTCGTCAGAGGTGGA
Internal bar code:CTGTCTTTGAGAAGGGGGCTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:272
LEAP-Seq percent confirming:28.5714
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:ATGGGATGGTGTGTATGCCG
Suggested primer 2:AACAGGGTCCACATCACGTC