| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.071321 |
| Chromosome: | chromosome 12 |
| Location: | 6544125 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g538150 | (1 of 1) K05019 - chloride channel, nucleotide-sensitive, 1A (CLNS1A) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGTAGGTCTCGGTCCTCGTGGCGGTTGGACGAAGCGAAGCTTGCGTGA |
| Internal bar code: | ATCCCTCTCGGTCAGGGGAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 681 |
| LEAP-Seq percent confirming: | 78.125 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCGTCTTGGAGGTCTCAT |
| Suggested primer 2: | CTGCGTCATAACTGCTGTGC |