| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.071322 |
| Chromosome: | chromosome 5 |
| Location: | 1884281 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g235850 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCAATCAAGCAATGGAAATGATTGTAGTAGAGGGGAAGAAGGGTCATG |
| Internal bar code: | ATGGCGCATCAAGTGTGTCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1355 |
| LEAP-Seq percent confirming: | 3.26087 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 89 |
| LEAP-Seq n unique pos: | 92 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGCGTGTGTCATTGATTC |
| Suggested primer 2: | AGAGAGCAGCGCTGATTGTT |