Insertion junction: CLIP2.071326_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.071326
Chromosome:chromosome 16
Location:7019186
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g673729 CPN11 Chaperonin 11, chloroplastic; (1 of 1) PTHR10772:SF13 - CHLOROPLAST CHAPERONIN 10 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGTCCAACGAAACGCGGAAGCAAACTTGCTGCATCAACGATTTCCATG
Internal bar code:AGTATATCTAGTATCGTGATTG

Confirmation - LEAP-Seq

LEAP-Seq distance:689
LEAP-Seq percent confirming:7.52688
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:172
LEAP-Seq n unique pos:186

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCGAATTTCCAGCATGAT
Suggested primer 2:CGACCCAAACTAGGTGCTGT