| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.071327 |
| Chromosome: | chromosome 12 |
| Location: | 9314334 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g542150 | (1 of 3) PF14108 - Domain of unknown function (DUF4281) (DUF4281) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCGCACACATCCAGCATACACATACACATGTACATACACATACACATA |
| Internal bar code: | GGCTTCCCAGCTTCGTAAAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 51 |
| LEAP-Seq percent confirming: | 3.84615 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAATCGTTGGGACCAGGCA |
| Suggested primer 2: | GCCCAGACCCATCCATTTCA |