| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.071338 |
| Chromosome: | chromosome 7 |
| Location: | 3314550 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g334700 | ATG101 | (1 of 1) PF07855 - Protein of unknown function (DUF1649) (DUF1649); Autophagy-related protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCCGTTCCAGCTCTCAACACCCTGGCCCGTGCCAGCTTTCTCCCGTAC |
| Internal bar code: | ATGGAAAGAAGACGGTCATATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3930 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTGTAAAGGTTGTGCGC |
| Suggested primer 2: | TTCACGTCGTGTCCAGTCAG |