| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.071343 |
| Chromosome: | chromosome 8 |
| Location: | 456058 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358900 | (1 of 3) 2.4.2.8 - Hypoxanthine phosphoribosyltransferase / Transphosphoribosidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGGGCTGCTGTCGACACGGTGCATTCTGGTGCCGTGTTGCCCCACGG |
| Internal bar code: | TCTTAACCCGATTCAGAGGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 193 |
| LEAP-Seq percent confirming: | 16.6667 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACTCACACATACGCACGT |
| Suggested primer 2: | CGGTACTTCTCGGCGTAGTC |