| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.071349 |
| Chromosome: | chromosome 9 |
| Location: | 6747590 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g417200 | (1 of 1) K15030 - translation initiation factor 3 subunit M (EIF3M) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTAGACATTCCTCCTTGGTGCTAATGTCTGGGGCGAGACTTTGTTGGC |
| Internal bar code: | CCTTCGGGACAGTTACGGCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5196 |
| LEAP-Seq percent confirming: | 76.6129 |
| LEAP-Seq n confirming: | 95 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 124 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTAGAGGGGCGAGTAAGCA |
| Suggested primer 2: | TCCGCATATTCGTACCACCG |