| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.071352 |
| Chromosome: | chromosome 8 |
| Location: | 372385 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358577 | (1 of 8) PF00023 - Ankyrin repeat (Ank) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTATATGGGCGTTAGAGCGATCTACGTGGTGGGTGAATTGCTGGCATA |
| Internal bar code: | TGAGGGGAACCAGAGTCATGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 321 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCTCGAGATTGAGAGCCA |
| Suggested primer 2: | CCCGCAATCTACAGTGAGGG |