| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.071355 |
| Chromosome: | chromosome 5 |
| Location: | 730466 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g245450 | UMM4,COQ5A | (1 of 1) K06127 - 2-methoxy-6-polyprenyl-1,4-benzoquinol methylase (COQ5); Ubiquinone/menaquinone biosynthesis methyltransferase | 3'UTR |
| Cre05.g245500 | FAP175,ANK24 | (1 of 1) PTHR24133//PTHR24133:SF21 - FAMILY NOT NAMED // ANKYRIN REPEAT-CONTAINING PROTEIN; Ankyrin Repeat Flagellar Associated Protein 175 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCAGCTTGGTCCAGCGTGCATGTTGTTTTATTCAAACGGGCTGCTGGG |
| Internal bar code: | GGATTTCGTGTTTGGTTTCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 524 |
| LEAP-Seq percent confirming: | 20.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACCCAGCAAAAACGACCC |
| Suggested primer 2: | AACACACCCGGATGATGCAT |