| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.071357 |
| Chromosome: | chromosome 7 |
| Location: | 5750951 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g353100 | (1 of 4) PTHR10229//PTHR10229:SF0 - GTP-BINDING PROTEIN HFLX // GTP-BINDING PROTEIN 6-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCGGTGGAATATTCGGCTGGCGGGCTGCGGTGCTAGTAGTAAACCTGT |
| Internal bar code: | CATAATGAACCGAAGTTCGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4242 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCGTGGAGTTATCGCATA |
| Suggested primer 2: | TGTTGACAGTGATCTGCGCT |