| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.071361 |
| Chromosome: | chromosome 1 |
| Location: | 6693634 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g047750 | RPL18a,RPL18A | (1 of 1) K02882 - large subunit ribosomal protein L18Ae (RP-L18Ae, RPL18A); Cytosolic 80S ribosomal protein L18a | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGAGCGTTGCCCTCTTGCAACGGGTACTGGACGAGAATGACCTGCCGC |
| Internal bar code: | TGGCGCTCTGGACACATGATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2437 |
| LEAP-Seq percent confirming: | 94.2857 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGACAGGCCAATGTATTG |
| Suggested primer 2: | TTGGTGTAGGTTACGGTGGC |