| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.071362 |
| Chromosome: | chromosome 6 |
| Location: | 2944129 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273400 | TMC2 | putative tRNA (cytosine-5)-methyltransferase | 3'UTR |
| Cre06.g273413 | (1 of 1) K14835 - ribosomal RNA methyltransferase Nop2 [EC:2.1.1.-] (NOP2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTGGTATGCGTTCTTTGGTGATGCTGAGAGGCGAGCTGTCGGTGGCTG |
| Internal bar code: | AGATGTCACCATCTGCGGCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 152 |
| LEAP-Seq percent confirming: | 28.125 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACATCGCAGCACATAGTG |
| Suggested primer 2: | GTGCGAGTGAGGAAGGTGAA |