| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.071369 |
| Chromosome: | chromosome 15 |
| Location: | 201273 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g634701 | DIV20,RECQ4 | (1 of 1) K10730 - ATP-dependent DNA helicase Q4 [EC:3.6.4.12] (RECQL4); RecQ-class helicase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAATCATATGAGTAATATACAAAACTGAGTGACCGAGCGATGGAAGAGCC |
| Internal bar code: | CATGATAGGATCAAAGAATGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 386 |
| LEAP-Seq percent confirming: | 7.84314 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGATGCATGCACGCAGTAG |
| Suggested primer 2: | TAGTACCGCCTTGGCTCTCT |