| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.071371 |
| Chromosome: | chromosome 9 |
| Location: | 1874398 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397550 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCAACGTCATCTCTCGGACTTAGTCGGGGTCGGTTCGTACAGGCACGG |
| Internal bar code: | GCGAAGGCATCCCTTGAGGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1464 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAGTGTACCGTGCGTGAG |
| Suggested primer 2: | AGTGCAGGGACATGTACGTG |