| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.071373 |
| Chromosome: | chromosome 1 |
| Location: | 1512156 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g008150 | RPK1 | RNA-polymerase-subunit kinase; (1 of 1) IPR000719//IPR001932//IPR002290//IPR011009 - Protein kinase domain // PPM-type phosphatase domain // Serine/threonine/dual specificity protein kinase, catalytic domain // Protein kinase-like domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGGCTGGACCTGCCTGCCTAGCCGCGCCTTTGTGTGTGCCTGTGTGGT |
| Internal bar code: | CTCAATACCTCTGGGTGTTATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1073 |
| LEAP-Seq percent confirming: | 81.1594 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCTTGATTGGACCTCCT |
| Suggested primer 2: | GAACGTGTATGGGGGATGCA |