| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.071376 |
| Chromosome: | chromosome 1 |
| Location: | 4106689 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g027300 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTATGTGTGACCCATTTGCACACCACAGGTTTGACCACCCGCGCGAGT |
| Internal bar code: | CGGGTGAAGTTATCCTTCACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 635 |
| LEAP-Seq percent confirming: | 73.3333 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGTTCATGGGCTGGATGG |
| Suggested primer 2: | GGTATAGATGGCACTGCGCT |