| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.071379 |
| Chromosome: | chromosome 3 |
| Location: | 3824796 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g170001 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTACGATGCTGCGGGCTCCCGCCATCAATATGGAAGGACTCCTCAGGCG |
| Internal bar code: | TGTACTGCATAGTTGATGTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2923 |
| LEAP-Seq percent confirming: | 92.6471 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGACGTCGAAACCACCTG |
| Suggested primer 2: | GGTGTTGTGCATGCATGTGT |