| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.071380 |
| Chromosome: | chromosome 17 |
| Location: | 6755799 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g746547 | (1 of 6) PF07716 - Basic region leucine zipper (bZIP_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCATTGAACCCTGCACTTTTACATTCCATGCTCTTTTACTCGCTTCGC |
| Internal bar code: | TACGTGCTTGAGTAGGATTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1464 |
| LEAP-Seq percent confirming: | 90.4762 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGATGGGCATGGGAGAC |
| Suggested primer 2: | ATGTGTCCAGGAATGCAGGG |