| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.071383 |
| Chromosome: | chromosome 8 |
| Location: | 885261 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g361500 | mS26,MRPS26 | (1 of 1) PF14943 - Mitochondrial ribosome subunit S26 (MRP-S26); Mitochondrial ribosomal protein S26 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCTGCGGGATGAGGCGTCGTGAGTGACTCGCGAGGAATGCAGGTGCCA |
| Internal bar code: | CCACGGCACTGTCCAGCGGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1033 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCACTCAAAGCAAGCTT |
| Suggested primer 2: | GCTGGCTCCAGATTTCAGGT |