| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.071385 |
| Chromosome: | chromosome 14 |
| Location: | 3481434 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630550 | DEG1B,DEG13,DEG12 | Deg protease; (1 of 3) PTHR22939//PTHR22939:SF81 - SERINE PROTEASE FAMILY S1C HTRA-RELATED // PROTEASE DO-LIKE 1, CHLOROPLASTIC | outside_mRNA |
| Cre14.g801657 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGGGGAGCTGGCCATTCCGCAACATCGGTGGCAATGTCAGAATACTT |
| Internal bar code: | GATTGAATGATTGGAAGTTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 575 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGAGGTTGCCTTAAACGG |
| Suggested primer 2: | CGCACCTTTGTCCAAGTTGG |