| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.071392 |
| Chromosome: | chromosome 9 |
| Location: | 1098005 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401750 | (1 of 3) PTHR10696 - GAMMA-BUTYROBETAINE HYDROXYLASE-RELATED; Similar to conserved hypothetical protein in bacteria | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGACCTCTAGGCGGTCGCCCAGCCGCAGGCGCATGCAGTACAGAAGGT |
| Internal bar code: | TACAAGACTTGCAACAAAACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1266 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTAATGAATGCTGTGGGCG |
| Suggested primer 2: | CACCGCAACCACGAATTTGT |