| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.071401 |
| Chromosome: | chromosome 17 |
| Location: | 3166640 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g720950 | SRD3 | 3-oxo-5-alpha-steroid 4-dehydrogenase family protein; (1 of 1) 1.3.1.94 - Polyprenol reductase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATGGCAGTGAGGAAAGCCATGTGGAGAGCGTGGAAGAGTGGCCCCATG |
| Internal bar code: | CGTGGGGCACTGCCCGGTTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 955 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTTGAGGGTCAGCTGAG |
| Suggested primer 2: | AGGAAGCGGAGGTAGGTCAT |