| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.071405 |
| Chromosome: | chromosome 12 |
| Location: | 9830260 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g546877 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGAATTTAGCTACAACAACGGCATTGCCAGGCGTTGCAGGGCAAGCAAG |
| Internal bar code: | GTGGGAGTACTTGTGAAATCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1828 |
| LEAP-Seq percent confirming: | 53.8462 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCTTGGTATCAACAGGCC |
| Suggested primer 2: | GTAGCAGATTTCGCGGCTTG |