| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.071419 |
| Chromosome: | chromosome 14 |
| Location: | 1980045 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g620750 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTGCCTCCTTATCTCCTCACGCATGTGTACACATTTACGTGCGCCCAC |
| Internal bar code: | AAGAAAGTTTCTGATTGAGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2294 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGGTCCGACAGTGATCTA |
| Suggested primer 2: | AATGTCGAGCTACTACCGCG |