| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.071422 |
| Chromosome: | chromosome 6 |
| Location: | 909616 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g255950 | (1 of 1) K14553 - U3 small nucleolar RNA-associated protein 18 (UTP18) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTACAAATAACGGCCCGTCGGCTGTGAACGGATTCTGAGCTGTCTCCG |
| Internal bar code: | GAGTTGTGCCATATAATTGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1748 |
| LEAP-Seq percent confirming: | 95.2381 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCTGTTGCGGATTCTGC |
| Suggested primer 2: | TCGTAAGAGCCCACCCCATA |