Insertion junction: CLIP2.071433_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.071433
Chromosome:chromosome 1
Location:4170688
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre01.g027900 (1 of 1) K17338 - receptor expression-enhancing protein 1/2/3/4 (REEP1_2_3_4); similar to HAV22 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGAGACGCCATCTTTTTTCAGTTTCAGTATCCGACCCACCAGAAAATGC
Internal bar code:TGGTGAGTCCTGGTCGTACGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:263
LEAP-Seq percent confirming:10.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GTTCCCAGCCTACATGACCC
Suggested primer 2:CACCACGAACAACAGCTTGG