| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.071449 |
| Chromosome: | chromosome 13 |
| Location: | 3465862 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g587350 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGATGTCAACATACACTTGCGGCCGCGCCCCCTTGTGCGCCTCTAGCAC |
| Internal bar code: | ATCGGGGGGAGGCTTGGCAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2865 |
| LEAP-Seq percent confirming: | 5.40541 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGAAGCTGAGCGTGCAAC |
| Suggested primer 2: | ATGTCGTCTCGCAACCTGTT |