| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.071450 |
| Chromosome: | chromosome 14 |
| Location: | 2962046 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g801644 | (1 of 1) K02434 - aspartyl-tRNA(Asn)/glutamyl-tRNA(Gln) amidotransferase subunit B (gatB, PET112) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCAGGCCGGACGTCCCGTGGCGCATGAACTTGTAAATAATCAAAGTAA |
| Internal bar code: | TTCTCGGCCTCTCGCAACACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 199 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGCGTGATGCTTGAGAGT |
| Suggested primer 2: | CCTGCCCCTGTAGACAAGTG |