| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.071452 |
| Chromosome: | chromosome 6 |
| Location: | 5327050 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g284850 | (1 of 1) K03155 - timeless (TIMELESS) | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTTTTCCGAGAGCTCCCGGAACTTGAGCCCCATCAGTTTGAGCTGACG |
| Internal bar code: | TTGTACTAATATACATTTGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1081 |
| LEAP-Seq percent confirming: | 71.4286 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTGTGGAGTGACGACGAG |
| Suggested primer 2: | CCCATGAGTCCATGCTTGGT |