| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.071452 |
| Chromosome: | chromosome 16 |
| Location: | 6132155 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677150 | (1 of 2) 2.4.1.122 - Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase | 3'UTR | |
| Cre16.g801986 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAACAAGATTACAACACGGCTTGGCCTGTTGACCAGTGGGGACTGCCC |
| Internal bar code: | GTGTCCCCGTAATTTTACCCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1874 |
| LEAP-Seq percent confirming: | 97.4359 |
| LEAP-Seq n confirming: | 76 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 78 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAGTGGGACTTGACGACG |
| Suggested primer 2: | GGGGGCGTGACTATGAGATG |