Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.071456 |
Chromosome: | chromosome 17 |
Location: | 6469660 |
Confidence (%): | 63 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g744297 | (1 of 1) IPR005181//IPR013830 - Acetylesterase, domain of unknown function DUF303 // SGNH hydrolase-type esterase domain | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCGTGTTGTACTTGCCCGGCCGGCCCAACGCCCTCAACGGCACCACTG |
Internal bar code: | CCCGCTTCTCCGTCCTAAACAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 750 |
LEAP-Seq percent confirming: | 50.9804 |
LEAP-Seq n confirming: | 26 |
LEAP-Seq n nonconfirming: | 25 |
LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGAAGGCTGGCTGTAGTGTG |
Suggested primer 2: | AGGGGAGAAATGCAAGACGG |