Insertion junction: CLIP2.071459_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.071459
Chromosome:chromosome 2
Location:3589027
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g800214 (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATTAAAAGCCCTCGGTGTGTTCCTGGTCGAACCTCTCTGACATGAGCAG
Internal bar code:TATTTTAGCAATATTGTCAGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1342
LEAP-Seq percent confirming:82.3529
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:AGACGAATGAGGTGCAGGTG
Suggested primer 2:TAAAATGTACACGGGGCCCC