| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.071480 |
| Chromosome: | chromosome 15 |
| Location: | 1154871 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g639200 | (1 of 8) 2.4.1.5 - Dextransucrase / Sucrose 6-glucosyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAATGCAAGCGATTATGTGTAGACCCCTCCATGCTCCAAGCTCACGTGG |
| Internal bar code: | CTTGGACTAGACATCGTGCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2040 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 57 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTGCGTGAGTTGAGCTGC |
| Suggested primer 2: | TTGACGACTGGCTGAACTCC |