Insertion junction: CLIP2.071484_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.071484
Chromosome:chromosome 2
Location:6575769
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g389450 (1 of 1) IPR000104//IPR000504//IPR003613//IPR012677//IPR013083//IPR016024//IPR020683 - Antifreeze protein, type I // RNA recognition motif domain // U box domain // Nucleotide-binding alpha-beta plait domain // Zinc finger, RING/FYVE/PHD-type // Armadillo-type fold // Ankyrin repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATTGTTCCTAGGACGCTGCGTTGTCAACTATCTATGCATGGCATGCACC
Internal bar code:CTGGGCGTTGCGGTATCTTTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:632
LEAP-Seq percent confirming:92.3077
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:ACAGCAACAGCAACACCTCT
Suggested primer 2:CATGCTACGCCACATGATGC