| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.071496 |
| Chromosome: | chromosome 13 |
| Location: | 1099372 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g569200 | SRP54 | (1 of 1) PTHR11564//PTHR11564:SF5 - GTPASE CONTAINING FAMILY OF SIGNAL RECOGNITION PARTICLE PROTEINS // SIGNAL RECOGNITION PARTICLE 54 KDA PROTEIN; 54kDa Subunit of the Signal Recognition Particle | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATTGCCGGTGCGTCGTAGTGCACACGCACCGTGCTTTGGCAGACGTGG |
| Internal bar code: | AGTACGAGTTAGTTTGCGATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1912 |
| LEAP-Seq percent confirming: | 23.8095 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGCGATGTGCTTTCTTAG |
| Suggested primer 2: | CGATCGACCTCTCTTCCTGC |