| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.071505 |
| Chromosome: | chromosome 10 |
| Location: | 4874140 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g453600 | DES,FAD13 | (1 of 2) PTHR19353:SF30 - FATTY ACID DESATURASE 2; Fatty acid omega 13 desaturase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCGCCGTCCTTGCCGACCAGGTTGGTGTAGGAGTGGTGCAGGATGTTG |
| Internal bar code: | ATGAGCCTACCTTGAAGCCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 470 |
| LEAP-Seq percent confirming: | 4.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCCCTTCCTTACACACCG |
| Suggested primer 2: | AAGGCCACGCTCATCATGAA |