Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.071506 |
Chromosome: | chromosome 9 |
Location: | 5871028 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g411800 | outside_mRNA |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTCACCAACCTGCCTTGACACTGTCCCATCGCACAGCCTTTGTTTGCA |
Internal bar code: | CTTACAAATGTGGAGCTCTCAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1375 |
LEAP-Seq percent confirming: | 42.5 |
LEAP-Seq n confirming: | 17 |
LEAP-Seq n nonconfirming: | 23 |
LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCCGCCCAACTTCATGTTCA |
Suggested primer 2: | CGCTCCTAAGTGTCGGAGTC |