| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.071510 |
| Chromosome: | chromosome 12 |
| Location: | 301327 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g485500 | (1 of 1) PTHR11757:SF12 - PROLYL ENDOPEPTIDASE-LIKE | CDS | |
| Cre12.g485550 | (1 of 1) 4.1.3.4 - Hydroxymethylglutaryl-CoA lyase / HMG-CoA lyase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACAAGCTTTGTCTGGCATTTCCACATGCTCTCATGCAAGTACTTCTT |
| Internal bar code: | ATGGACTCCTAATATGTTCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1894 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTTTACCGCAGCGTGTCC |
| Suggested primer 2: | CGCGTAGGGTGCATAAAACG |