| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.071513 |
| Chromosome: | plastome |
| Location: | 19857 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802271 | ChreCp009,2717052,chlL | light-independent protochlorophyllide reductase iron-sulfer ATP-binding protein; (1 of 1) K04037 - light-independent protochlorophyllide reductase subunit L (chlL) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTTCTTATATAATACTGCGTCAGATATAATACTGCGTAAGCAGAAACT |
| Internal bar code: | ATCGTTTTTATTAGTTTCCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 372 |
| LEAP-Seq percent confirming: | 52.6316 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGGACGTTTGAAACCACC |
| Suggested primer 2: | CCCGCTAAACGCAATGGATG |